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AZDye™ 350 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AZDye™ 350 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AZDye™ 350 is a water-soluble moderately photostable, blue-fluorescent probe optimally excited by the 350 nm laser line. It is routinely used for generation of stable signal in imaging and flow cytometry. The brightness and photostability of blue dyes are best suited to direct imaging of high-abundance targets.
AZDye™ 350 is structurally identical to Alexa Fluor® 350. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes sharing a coumarin dyes core, including DyLight® 350, AMCA, Alexa Fluor® 350 and CF® 350 Dye.
DyLight® and Alexa Fluor® are registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademarks of Biotium Inc.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 346/445 nm |
| Extinction Coefficient | 19,000 |
| Spectrally Similar Dyes | Alexa Fluor® 350, CF® 350, DyLight 350, AMCA |
| Molecular weight | 722.14 (protonated) |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Grey to yellow solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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