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AZDye™ 430 DBCO dye reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AZDye™ 430 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
AZDye™ 430 is a bright, and photostable, green-fluorescent probe optimally excited near its absorption maximum at 432 nm. Its emission peaks at 539 nm is pH independent over a wide pH range. The brightness and photostability of blue dyes are well suited to direct imaging of moderate to high abundance targets.
AZDye™ 430 is structurally identical to Alexa Fluor® 430. Its absorption/emission spectra is a perfect match to spectra of many other structurally similar dyes, including Alexa Fluor® 430 and CF®430 Dye.
AZDye™ 430 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 430/537 nm |
| Extinction Coefficient | 15,000 |
| Spectrally Similar Dyes | Alexa Fluor® 430, CF® 430 |
| Molecular weight | 761.81 |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Yellow solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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