AZDye™ 488 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. AZDye™ 488 Biotin is a water soluble reagent and its green fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.
AZDye™ 488 is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of blue dyes are best suited to direct imaging of low-abundance targets.
AZDye™ 488 is structurally identical to Alexa Fluor® 488. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine 110 core, including DyLight® 488, Alexa Fluor® 488, and CF® 488A.
DyLight® and Alexa Fluor® are a registered trademark of Thermo Fisher Scientific.
| Unit Size | 1 mg, 5 mg |
|---|---|
| Abs/Em Maxima | 494/517 nm |
| Extinction Coefficient | 73,000 |
| Flow Cytometry Laser Line | 488 nm |
| Microscopy Laser Line | 488 nm |
| Spectrally Similar Dyes | FAM, Alexa Fluor® 488, Atto™ 488, CF® 488A Dye, DyLight® 488 |
| Molecular weight | 935.00 (protonated) |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Orange to light red solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
Applicable patents and legal notices are available at legal notices.
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