AZDye 532 DBCO (CCT-1282)

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This product was previously sold as 1046 from Fluoroprobes.

Description
Specifications
Abs/Em Spectra
Documents

Description

AZDye™ 532 DBCO is an azide reactive probe that can be used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern AZDye™ 532 DBCO is an ideal alternative to copper requiring fluorescent alkynes.

AZDye™ 532 DBCO is ideally suited for the frequency-doubled Nd:YAG laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of AF 532 dyes are well suited to direct imaging of low-abundance targets.

AZDye™ 532 is spectrally almost identical to many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.

Specifications

Unit Size	1 mg, 5 mg, 25 mg
Abs/Em Maxima	532/554 nm
Extinction Coefficient	78,000
Flow Cytometry Laser Line	532 nm
Microscopy Laser Line	532 nm
Spectrally Similar Dyes	Alexa Fluor® 532, Atto™ 532, CF® 532 dyes
Molecular weight	872.92
CAS	N/A
Solubility	Water, DMSO, DMF
Purity	>95% (HPLC)
Appearance	Orange to light red solid
Storage Conditions	-20°C. Desiccate
Shipping Conditions	Ambient temperature