AZDye 647 Azide (CCT-1299)

Description

AZDye™ 647 Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AZDye™ 647 over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

AZDye™ 647 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.

AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. AZDye™ 647 Azide can be used a less expensive alternative to Alexa Fluor® 647 Azide.

Specifications

Abs/Em Spectra

Documents

Selected References

1. Heybrock, S., et al. (2021). S-palmitoylation determines TMEM55B-dependent positioning of lysosomes. J Cell Sci., 135 (5), jcs258566. [PubMed]
2. Lee, M.T., et al. (2020). Dietary sphinganine is selectively assimilated by members of themammaliangut microbiome. J Lipid Res . [PubMed]