AZDye 680 is a bright and photostable near-IR dye that is spectrally and structurally identical to Alexa Fluor® 680 Dye. AZDye 680 is often used for small animal imaging applications in the 700 nm wavelength and provides excellent clearance profiles in animals. Flow cytometry is another common application for this dye. AZDye 680 ideally suited for the 633 nm laser line. This near-IR fluorescent dye is water soluble and pH-insensitive from pH 4 to pH 10. AZDye 680 molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals.
The NHS ester (succinimidyl ester) of AZDye 680 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
Unit Size | 1 mg, 5 mg, 25 mg, 100 mg |
---|---|
Reactivity | Primary amine |
Abs/Em Maxima | 678/701 nm |
Extinction coefficient | 185,000 cm-1M-1 |
Solubility | Water, DMSO, DMF |
Spectrally similar dyes | Alexa Fluor® 680, DyLight® 680, IRDye® 680 |
Molecular weight | 955.90 |
Storage Conditions | -20°C. |
Shipping Conditions | Ambient temperature |
[protein-labeling-calculator]
Stay in the Loop
Join Our Online Community
The latest news, tips, and product releases delivered conveniently to your inbox.
We’re social scientists! Let’s connect.
Together we breakthroughTM
©Vector Laboratories, Inc. 2023 All Rights Reserved.
How do I Request a Quote?
To request a quote for products: