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Methyltetrazine-activated Cy5 probe that reacts with TCO-containing compounds via an Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics (k > 800 M-1 -1) and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 649/671 nm |
| Extinction Coefficient | 250,000 |
| Flow Cytometry Laser Line | 633 or 635 nm |
| Microscopy Laser Line | 633 or 635 nm |
| Spectrally Similar Dyes | Alexa Fluor® 647, Atto™ 647, CF® 647 Dye, DyLight® 649 |
| Molecular weight | 934.10 |
| CAS | N/A |
| Solubility | Water, DMSO, DMF, MeOH |
| Purity | >95% (HPLC) |
| Appearance | Blue solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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