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SY-21 Azide (QSY®-21 equivalent) is a nonfluorescent acceptor dye often used for preparation of peptide and oligonucleotide FRET probes. A broad and intense quenching range from 580 nm to 680 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with fluorescent dyes at 580 nm to 680 nm, such as Fluorescein, Alexa Fluor® 568, 594, 633, 647, TAMRA, ROX, Texas Red and Cy5.
SY-21 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures.
| Unit Size | 5 mg, 25 mg, 100 mg |
|---|---|
| Abs/Em Maxima | 661 nm |
| Extinction Coefficient | 90,000 cm-1M-1 |
| Molecular weight | 883.06 |
| Spectrally compatible dyes | Alexa Fluor® 568, 594, 633, 647, TAMRA, ROX, Texas Red, Cy5. |
| CAS | N/A |
| Solubility | DMSO, DMF |
| Appearance | Dark blue solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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