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Universal Black 3 Quencher (UBQ-3, Black Hole Quencher®-3 (BHQ®-3) equivalent) is a dark, far-red nonfluorescent acceptor dye with no native emission. UBQ-3 is often used as an acceptor for preparation of fluorogenic dual-labeled probes, qPCR probes, and for other fluorescence-quenched probe applications. A broad and intense quenching range from 620-730 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with far-red to near-IR emitting dyes such as Cy5, Cy5.5, Alexa Fluor® 633, 647, 700, IRDye® 650, 680 and 700.
UBQ-3 is structurally identical to BHQ®-3.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. The labeling occurs most efficiently at pH 7-9 and forms a stable, covalent amide bond.
| Unit Size | 5 mg, 25 mg, 100 mg |
|---|---|
| Reactivity | Primary amine |
| Abs/Em Maxima | 615 nm |
| Extinction coefficient | 40,700 cm-1M-1 |
| Solubility | DMSO, DMF |
| Spectrally similar dyes | Cy5, Cy5.5, Alexa Fluor® 647, 680, 700, IRDye® 650, 680, 700 |
| Molecular weight | 664.76 |
| Storage Conditions | -20°C. |
| Shipping Conditions | Ambient temperature |
[protein-labeling-calculator]
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