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5(6)-Carboxyrhodamine 110 NHS Ester (also known as Rhodamine Green™ Carboxylic Acid, Succinimidyl Ester, Hydrochloride or 5(6)-CR 110, SE) is the nonsulfonated analog of the Alexa Fluor® 488 dye. The amine-reactive 5(6)-Carboxyrhodamine 110 NHS Ester can be used to create bright and photostable green-fluorescent bioconjugates with excitation/emission maxima ~502/527 nm.
The conjugates of this dye often used for Fluorescence Correlation Spectroscopy (FCS). For many applications, 5(6)-Carboxyrhodamine 110 NHS Ester is preferred over 5-(6)-carboxyfluorescein NHS ester or FITC because of its exceptional photostability and fluorescence insensitivity to pH (4-9).
| Unit Size | 5 mg, 25 mg, 100 mg |
|---|---|
| Reactivity | Primary amine |
| Abs/Em Maxima | 502/527 nm |
| Extinction coefficient | 76,000 cm-1M-1 |
| Solubility | DMSO, DMF |
| Spectrally similar dyes | Alexa Fluor® 488, DyLight® 488, Fluorescein, Oregon Green 488 |
| Molecular weight | 471.43 |
| Storage Conditions | -20°C. |
| Shipping Conditions | Ambient temperature |
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