AZDye™ 430 NHS Ester is a water-soluble, green-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye™ 405 conjugates are pH-insensitive from pH 4 to pH 10.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with AZDye™ 430 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.
Unit Size | 5 mg, 25 mg, 100 mg |
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Reactivity | Alkynes, cyclooctynes |
Abs/Em Maxima | 430/539 nm |
Extinction coefficient | 15,000 cm-1M-1 |
Solubility | Water, DMSO, DMF |
Spectrally similar dyes | Alexa Fluor® 430 |
Molecular weight | 600.56 |
Storage Conditions | -20°C. |
Shipping Conditions | Ambient temperature |
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