BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium) (SK-5400)

Description

BCIP/NBT AP Substrate Kit forms an indigo, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.

Features:

Greater sensitivity than conventional substrates
Consistent and reliable
Ideal for IHC/ICC/ISH and blots
Fluorescent
Heat Stable
Permanent mounting
No wait times for mixing and dissolving powders or tablets
Sufficient reagents to produce 200 ml of working solution
Excitation: 645-685 nm*
Emission: peaks at 823 and 855 nm*

*Biotechniques (2007) 42:756-759 and BioTechniques (2014) 57:254-256

Specifications

More Information
Unit Size	1 kit
Applications	Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s)	Alkaline Phosphatase
Substrate Reaction Product	Precipitating Reaction Product
Chromogen Color	Indigo
Color of Fluorescence	Far Red
Microscopy	Brightfield, Fluorescence, Spectral Imaging
Additional Substrate Properties	Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
Mounting	Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)
Safety Title	WARNING: Reproductive Harm - www.P65Warnings.ca.gov

Kit Contents:

4 ml BCIP/NBT Reagent 1
3 ml BCIP/NBT Reagent 2
4 ml BCIP/NBT Reagent 3

Documents

Citations

Technical Information

This substrate can be used for developing blots or for in situ hybridization. At 20-30 minute development times, the sensitivity of this substrate is equivalent to the other alkaline phosphatase substrates. However, BCIP/NBT will continue to develop from several hours to overnight making it one of the most sensitive chromogenic substrates. This kit contains stock solutions in convenient dropper bottles.