Streptavidin, DyLight™ 594 (SA-5594-1)

Description
Specifications
Documents
Citations
Technical Information

Description

Features:

Can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry
Highly purified and possess very low non-specific binding properties
Extremely high affinity for biotin
Advantages of DyLight dyes include greater photostability, pH independence, and brighter fluorescence
Excitation at 592 nm
Emission at 617 nm
Color: Red

Specifications

Unit Size	1 mg
Applications	Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage	Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution	10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation	592 nm
Maximum Emission	617 nm
Recommended Storage	2-8 °C
Concentration	1.0 mg/ml active conjugate
Conjugate	DyLight 594
Color of Fluorescence	Red
Format	Concentrate

Documents

Citations

Technical Information

Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

DyLight dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.