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Streptavidin, fluorescein (FITC) can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Amplification of fluorescent signals can be easily achieved with our highly purified fluorescein labeled streptavidin.
Features:
| Unit Size | 1 mg |
|---|---|
| Applications | Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation |
| Recommended Usage | For diluting this product, we recommend a HEPES-, bicarbonate- or phosphate- buffered saline solution, approximately pH 8.2. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents. The recommended range of concentrations for staining of cells or tissues is 5-30 µg/ml. |
| Solution | 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. |
| Maximum Excitation | 495-500 nm |
| Maximum Emission | 514-521 nm |
| Recommended Storage | 2-8 °C |
| Concentration | 1.0 mg active conjugate/ml |
| Conjugate | Fluorescein |
| Color of Fluorescence | Green |
| Format | Concentrate |
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