Streptavidin, Peroxidase (Concentrate, for IHC) (SA-5004-1)

Description

Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of the peroxidase.  Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears.

Features:

  • Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
  • Horseradish Peroxidase Streptavidin is offered as a concentrate (1 mg/ml) or in a ready-to-use (R.T.U.), prediluted, stabilized solution in a dropper bottle

 

For horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:

Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots

Specifications

Unit Size1 mg
ApplicationsImmunohistochemistry / Immunocytochemistry, In situ hybridization
Recommended UsageMolar ratio active conjugate: 1.5. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. The recommended concentration range for use is 2-5 µg/ml.
Solution0.05 M HEPES, 0.1M Na2S04 pH 6.8, preservative
Recommended Storage2-8 °C, DO NOT FREEZE
Concentration1.0 mg/ml active conjugate
Detection Enzyme(s)Peroxidase
ConjugatePeroxidase
FormatConcentrate

Citations

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